Tuberin, a product of the tumor suppressor gene TSC2, is important in regulating cell proliferation and tumor development (1).
Mutation in either TSC1 or TSC2 is responsible for tuberous sclerosis complex (TSC), an autosomal dominant disorder (2).
Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3).
Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5).
Additionally, tuberin inhibits the mammalian target of rapamycin (mTOR), resulting in inhibition of p70 S6 kinase and activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation) and, thus, inhibition of translation (3, 6-7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of tuberin only when phosphorylated at Tyr1571. Does not detect tuberin phosphorylated at other sites. Species Crossreactivity: mouse and monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mk Mo
Immunogen：Synthetic phosphopeptide corresponding to residues around Tyr1571 of human tuberin. Species Sequence Homology: human (100%)
