84-1051-76　Stathmin 100ul　S7972-50

特徴

• Stathmin, a ubiquitously expressed microtubule destabilizing phosphoprotein, is upregulated in a number of cancers.
• The N-terminus of the protein is involved in promotion of tubulin filament depolymerization and contains multiple phosphorylation sites.
• Phosphorylation at these sites inactivates the protein and stabilizes microtubules.
• Ser16 phosphorylation by CaM kinase II and IV (1,2) increases upon entrance into the G2/M-phase and is involved in mitotic spindle regulation (3,4).
• Ser38 is a target for cdc2 kinase phosphorylation (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6).
• EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7).
• Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3’ and RB3’’.
• Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC12 cells (8).
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.