Phosphorylation of Tyr416 in the activation loop of the kinase domain upregulates the enzyme; phosphorylation of Tyr527 in the C-terminal tail by Csk renders the enzyme less active (2).
Src is a member of the Src family of protein tyrosine kinases.
This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets.
Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins.
Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor.
In addition, Src is thought to play a role in physiological/pathophysiological processes in the central nervous system.
It has also been reported that mice deficient in Src develop severe osteoporosis.
A total of eight membrane-associated protein tyrosine kinases have been identified within the Src gene family.
Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr whose roles include regulation of cytoskeletal organization, cell-cell contact, DNA synthesis, proliferation and differentiation of eukaryotic cells.
Elevated activity of the proto-oncogene Src has been found in many human cancers, most notably breast cancer.The highest levels of expression of the prototype member of this gene family, Src p60, are observed in platelets and in neuronal tissues.
With the fact that platelets have constitutively high levels of phosphotyrosine-containing proteins suggest that Src p60 may be important for platelet homeostasis.
Applications:Suitable for use in Western Blotting.
Other applications not tested.
Recommended Dilution:Western blotting: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects endogenous levels of Src only when Tyr527 is dephosphorylated. It may also crossreact with Yes, Fyn and Slk based on sequence similarity.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr527 of human Src.
