SHP2, also known as SHPTP2, Syp, PTP1D and PTP2C, is a ubiquitously expressed tyrosine-specific protein phosphatase.
It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death (1).
Activation of SHP 2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2).
In addition to its role in Gab1-mediated Erk activation, SHP 2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 p85 binding sites (3).
SHP 2 becomes phosphorylated at Tyr542 and Tyr580 in its C-terminus in response to growth factor receptor activation (4).
These phosphorylations are proposed to relieve basal inhibition and stimulate its tyrosine phosphatase activity (5).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human SHP-2 only when phosphorylated at Tyr542. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr542 of human SHP-2. MW: 72kD. Species sequence homology: monkey, chicken, Xenopus.
