It serves a scaffolding function in signaling for a variety of receptor tyrosine kinases.
Shc exists in p46, p52 and p66 isoforms.
These isoforms are produced by using alternative translation initiation sites or a differentially spliced message (1-3).
In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6).
Grb2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4).
Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects Shc only when phosphorylated at tyrosine 239/240. May crossreact with activiated EGF receptor protein. Species Crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr239/240 of human Shc (KLH coupled).
