The Ret proto-oncogene (c-Ret), a receptor tyrosine kinase, functions as a multicompetent receptor complex in conjunction with other membrane bound ligand-binding GDNF family receptors (aGFRs) (1).
The ligands for c-Ret have been identified as the glial cell line-derived neurotropic factor (GDNF) and its congeners neurturin, persephin and artemin (2-4).
Gene alterations in c-Ret have been found to associate with different diseases including papillary thyroid carcinoma, multiple endocrine neoplasma type 2A (MEN2A), MEN2B, familial medullary thyroid carcinoma and a congenital developmental defect called Hirschsprungís disease (1,3).
Tyr905 is located in the kinase domain of Ret and plays crucial roles in its catalytic and/or biological activities.
Substitution of phenylalanine for Tyr905 dramatically inhibits its autophosphorylation activity (5).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human Tyr905 phosphorylated Ret. Does not crossreact with other tyrosine-phosphorylated proteins. Species crossreactivity: Drosophila.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Dr Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr905 of human Ret.
