84-1051-69　Ras, GRF1 100ul　R1198-16

特徴

• Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors).
• Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2).
• Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2).
• Multiple PKA phosphorylation sites on Ras-GRF have been identified.
• Phosphorylation on the two major sites, serines 754 and 822, inhibits Ras-GRF activity (3).
• Carbachol (a muscarinic agonist)-induced phosphorylation on serine 916 is essential but not sufficient for maximal Ras-GRF activity (4).
• It has been reported that Ras-GRF1 shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000.
• Detects a band at ~155kD
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.