Protein tyrosine kinase Pyk2, also called CAKbeta, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1–4).
Pyk2 is predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system.
Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway.
It plays an important role in cell motility such as spreading and migration (5–7).
Specificity/Sensitivity: Pyk2 Antibody detects total levels of endogenous Pyk2.
Applications:Suitable for use in Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total Pyk2.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding the N-terminus of human Pyk2.
