Although protein kinase C (PKC) family members are involved in a number of cellular signal transduction processes, such as secretion, gene expression, proliferation and muscle contraction (1,2), many of its substrates remain unidentified.
Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC) and atypical PKCs (aPKC).
PKCalpha, beta I, beta II and gamma isoforms belong to cPKC (1).
When activated, cPKC isozymes phosphorylate substrates containing serine or threonine, with arginine or lysine at the -3, -2 and +2 positions, and hydrophobic amino acids at position +1 (1-3).
In order to identify potential PKC substrates, we have antibodies that recognize these motifs.
As shown by ELISA, Phospho-(Ser) PKC Substrate Antibody is specific to cPKC substrates containing phospho-serine.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50ELISA: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects phosphorylated serine residues in many cellular proteins at endogenous levels, when those phospho-serine residues are surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The antibody does not crossreact with non-phosphorylated serine residues; with phospho-threonine in the same motif; or with phosphoserine in other motifs.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Immunogen：Synthetic peptides corresponding to phospho-PKC substrate.
