A class of kinases that regulates an array of physiological processes and includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase, protein kinase C, Akt and RSK shares a substrate specificity characterized by arginine at position –3 relative to the phosphorylated Ser or Thr.
These are referred to as Arg-directed kinases or AGC-family kinases.
Phospho-(Ser/Thr) PKA Substrate Antibody provides a powerful new tool for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 for 4ºC with gentle shaking overnight.
ELISA (Peptide): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not recognize the nonphosphorylated PKA substrate motif.
