Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA).
PA is the precursor of the second messenger, diacylglycerol (DAG).
Two isoforms of PLD (PLD1 and PLD2) have been identified.
Both are regulated by protein kinases, small GTPases and Ca2+.
PLD1 is phosphorylated at Ser2, Ser561 and Thr147 by PKC.
Phosphorylation at Ser147 and Thr561 activates PLD1 activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Band is observed at ~120kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of human PLD1 phosphorylated at Thr147. Does not crossreact with phosphorylated PLD2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr147 of human PLD1.
