Phosphatidylcholine-specific phospholipase D (PLD) hydrolyzes phosphatidylcholine (PC) to produce choline and phosphatidic acid (PA).
PA is the precursor of the second messenger diacylglycerol (DAG).
Two isoforms of PLD (PLD1 and PLD2) have been identified.
Both are regulated by protein kinases, small GTPases and Ca2+ (1).
PLD1 is phosphorylated at serine 2, serine 561 and threonine 147 by PKC (2,3).
Phosphorylation at threonine 147 and serine 561 regulates PLD1 activity (3).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of human PLD1 only when phosphorylated at serine 561. Does not crossreact with phosphorylated PLD2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser561 of human PLD1.
