Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2), activating Akt, PKC isoenzymes p70 S6 kinase and RSK (3).
Through its effects on these kinases, PDK1 is involved in regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human PDK1 protein at 58-68kD. Species Crossreactivity: mouse, rat, monkey.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding the C-terminus of human PDK1 (KLH).
