Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system.
It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling and angiogenesis (1,2).
eNOS is regulated by phosphorylation at multiple sites.
The two most throughly studied sites are the activation site serine 1177 and the inhibitory site threonine 495 (3).
Several protein kinases including Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5).
In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation (6,7).
Applications:Suitable for use in Immunoprecipitation and Western Blot.
Other applications not tested.
Recommended Dilution:Immunoprecipitation: 1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of eNOS only when phosphorylated at threonine 495. Species Crossreactivity: Bovine and porcine. Reactivity is expected with human, mouse and rat due to 100% sequence homology with peptide used for immunogen.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Bo Hu Po
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr495 of human eNOS.
