N-methyl-D-aspartate receptor (NMDAR) mediates long-term potentiation and slow postsynaptic excitation, which play central roles in learning, neurodevelopment and neuroplasticity (1).
The receptor function can be regulated by the protein kinase/phosphatase complex associated with it (2).
PKC can phosphorylate the NR1 subunit (NMDAR1) of the receptor on Ser890/ Ser896 and PKA can phosphorylate NR1 on serine 897 (3).
The phosphorylation of NR1 by PKC decreases its affinity to calmodulin binding, thus preventing the inactivation effect of calmodulin on NMDAR (4).
The phosphorylation of NR1 by PKA probably counteracts the inhibitory effect of calcinurin on the receptor (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes overexpressed and endogenous NMDAR1 only when phosphorylated at serine 890. Species Crossreactivity: human, mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around serine 890 of human NMDAR1.
