The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1).
Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2).
p95/NBS1 is a protein with a Forkheadassociated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3).
The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 may function in the same biochemical pathway.
Recent research has shown that ATM interacts with and phosphorylates p95/NBS1 on Ser343 after IR (4–7).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human p95/NBS1 protein. Species crossreactivity: mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide (KLH coupled) corresponding to C-terminal residues of human p95/NBS1.
