84-1051-38　Met, phosphorylated (Tyr1349) 100ul　M3007-22

特徴

• Met is a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor).
• Met is a disulfide-linked heterodimer made of 45kD alpha and 145kD beta-subunits (1,2).
• The alpha-subunit andthe amino-terminal region of beta-subunit form the extracellular domain.
• The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity.
• Interaction ofMet with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase (3) and is critical for all of its known biological functions
• Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation.
• Phosphorylation of Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (4).
• Altered Met levelsand/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers.
• Met is an attractive therapeutic target and cancer diagnostic agent (5).
• Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
• Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.