MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1) via Akt-mediated MDM2 phosphorylation at Ser166 and Ser186 (2,3).
Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53 (4).
Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).
Specificity/Sensitivity: Phospho-MDM2 (Ser166) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 166.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects endogenous levels of MDM2 only when phosphorylated at serine 166.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser166 of human MDM2.
