Matrix metalloproteinase (MMP) is a family of proteinases that target many extracellular proteins, such as other type of proteinases, growth factors, cell surface receptors and adhesion molecules (1).
Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryo development and wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4).
MMP activity is correlated with cancer development (2).
One way of MMP regulation is controlling the overall proteinase expression at the transcription step (5).
Once synthesized, MMP exists in a latent proenzyme form.
The maximum MMP activity requires proteolytic cleavage of MMPs to generate active MMPs by release of the inibitory propeptide domain from the whole molecule (5,6).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunohistochemistry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects human full length (proenzyme, 92kD) and cleaved (active enzyme, 84kD) MMP-9.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues around 107-117 of human MMP-9.
