In response to cytokines, stress and chemotactic factors, MAP kinase–activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated.
It has been illustrated that MAPKAPK-2 is a direct target of p38 MAPK (1).
Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress.
However, only four of the residues—Thr25, Thr222, Ser272 and Thr334—are phosphorylated by p38 in an in vitro kinase assay (2).
Phosphorylation at Thr222, Ser272 and Thr334 seems to be essential for the activity of MAPKAPK-2 (2).
Thr25 is also phosphorylated by p42 MAPK in vitro and is not required for the activation of MAPKAPK-2 (2).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Flow Cytometry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total MAPKAPK-2 protein. Does not crossreact with MAPKAPK-3 or -5. Species Crossreactivity: Human, mouse, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：FC IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide (KLH coupled) in the C-terminal region of human MAPKAPK-2. Species sequence homology: hamster
