Peutz-Jeghers syndrome (PJS) is caused by mutation of a widely expressed protein kinase termed LKB1 (1,2) that is predicted to function as a tumor suppressor (1,2).
LKB1 is a 433aa protein possessing a kinase domain only distantly related to other mammalian kinases.
The amino-terminal noncatalytic domain comprises both a nuclear localization signal (3) and a putative cytoplasmic retention signal (4).
The mechanism by which LKB1 is regulated in cells is not known, nor have any of its physiological substrates been identified.
Recent studies have demonstrated that LKB1 is phosphorylated in cells at serines 31, 325 and 431, and at threonines 189, 336 and 366 (5,6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects overexpressed levels of LKB1 only when phosphorylated at threonine 189. Does not detect LKB1 phosphorylated at other sites.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr189 of human LKB1.
