The common beta-chain (beta-c) of the granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and IL-5 receptors is the major signaling subunit of these receptors coupling ligand binding to multiple biological activities (1-3).
Tyrosine phosphorylation of cytokine receptor common beta-chain is one of the first events in GM-CSF, IL-3 and IL-5 activation of their receptors and in the initiation of the signaling cascade (4).
Serine phosphorylation within the 14-3-3 binding sequence of the common beta-chain is also involved in GM-CSF, IL-3 and IL-5 receptor-specific functions (5,6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of total human cytokine receptor common beta-chain protein at ~100kD independent of phosphorylation. Species Crossreactivity: Mouse, rat, monkey, mink
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide derived from the C-terminus of human cytokine receptor common beta-chain.
