GRB7 family adaptor proteins consist of GRB7, GRB10 and GRB14, which contain an N-terminal proline-rich SH3 binding domain, followed by PH, PBS and SH2 domains.
Each member of the family has several splicing isoforms (1).
It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules such as Raf, Akt and Nedd4 (1,2).
Although it was originally reported that GRB10 is exclusively phosphorylated at serine residues (3), recent research has shown that GRB10 is also phosphorylated at tyrosine 67 by Src kinase family members (4).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human total GRB10 protein independent of phosphorylation at 76kD. Does not crossreact with GRB7 or GRB14.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to N-terminal residues of human GRB10 (KLH).
