GRB-associated binder (Gab) is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs), such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors.
Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane, undergoes phosphorylation and functions as a multiprotein assembling center (1-4).
Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5).
Phosphorylation of tyrosine 472 regulates its binding to p85 PI3 kinase (6,7).
Phosphorylation of tyrosines 307, 373 and 407 modulates the association to PLCgamma (8).
Phosphorylation of tyrosines 627 and 659 are required for the binding and activation of protein tyrosine phosphatase SHP2 (6,9).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of human Tyr307 phosphorylated Gab1. This antibody crossreacts with phosphorylated Gab2 and may also crossreact with phosphorylated Gab3. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr307 of human Gab1.
