FMS-related tyrosine kinase 3 (FLT3, also called Flk2), a member of the type III receptor tyrosine kinase family that includes c-Kit, PDGF and M-CSF receptors, is expressed on early hematopoietic progenitor cells, and supports growth and differentiation within the hematopoietic system (1,2).
FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream cellular targets (3).
The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4–6).
Tyrosine 591 is located in the juxtamembrane region of FLT3.
It may play an important role in regulation of FLT3 tyrosine kinase activity.
Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Nonglycosylated form of Flt3 shows a band at ~130kD.
Glycosylated Flt3 is seen at ~160kD
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous human FLT3 only when phosphorylated at tyrosine 591. Does not recognize other FLT family members. May crossreact with some tyrosine phosphporylated proteins. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr591 of human FLT3 (KLH).
