Erk5, also known as BMK1 (big mitogen-activated kinase 1), has a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the N-terminal kinase domain with Erk1/2.
However, the long C-terminal domain makes Erk5 a unique MAP kinase.
Erk5 is activated by a wide variety of extracellular signals, including tyrosine receptor kinases, G-protein-coupled receptors as well as osmotic and oxidative stress (1,2).
The direct upstream kinase of Erk5 is defined as MEK5 (1).
MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3-5).
Growing evidence indicates that the Erk5 pathway is involved in proliferation/differentiation regulation, oncogenesis and neuronal development (1,6-8).
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See E3452-59A1.
Specificity：Detects immunoprecipitated or transfected levels of human Erk5 phosphorylated at threonine 218/tyrosine 220. Crossreacts with phosphorylated Erk1 and Erk2. It does not crossreact with phosphorylated p38 MAPK or SAPK/JNK. Species Crossreactivity: mouse, monkey and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr218/Tyr220 of human Erk5
