Erk5, also known as BMK1 (big mitogen-activated kinase 1), has a TEY motif in the activation loop similar to Erk1/2, and shares high homology in the amino-terminal kinase domain with Erk1/2.
However, the long carboxy-terminal domain makes Erk5 a unique MAP kinase.
Erk5 is activated by a wide variety of extracellular signals, including tyrosine receptor kinases, G-protein-coupled receptors as well as osmotic and oxidative stress (1,2).
The direct upstream kinase of Erk5 is defined as MEK5 (1).
MEKK2, MEKK3 and Tpl-2 are all able to activate MEK5, depending on stimulus or cell background (3–5).
Growing evidence indicates that the Erk5 pathway is involved in proliferation/differentiation regulation, oncogenesis and neuronal development (1,6–8).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000; Mr ~115kDImmunoprecipitation: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Also available without BSA & Azide. See E3452-59C.
Specificity：Detects endogenous levels of total human Erk5 protein. Does not crossreact with Erk1 or Erk2. Species Crossreactivity: mouse, rat and monkey.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic peptide corresponding to human Erk5.
