84-1050-97　eEF2k, Phosphorylated (Ser366) (eEF2k (Eucharyotic Elongation Factor-2 kinase, eEF-2 kinase) 100ul　E2213-55

特徴

• Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1).
• eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3).
• It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress-or starvation-related conditions.
• eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis.
• This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38d at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.
• Applications:Suitable for use in Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.