Dynamin is a family of large GTPases that has been implicated in the formation of nascent vesicles of both endocytotic and secretory processes.
Dynamin plays an important role in the internalization of cell surface receptors, a process that attenuates the responsiveness of signal transduction.
It has been illustrated that dynamin interacts with signaling proteins such as Src, PLCgamma, PKC and G-proteins.
Dynamin is phosphorylated by PKC and Src.
Its phosphorylation may regulate the endocytosis of cell surface receptors.
Applications:Suitable for use in Western Blot, ELISA and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:30Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human total dynamin protein. Does not recognize the related dynamin-like protein DLP. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues surrounding Tyr231 of human Dynamin.
