C-Kit is the receptor tyrosine kinase for stem cell factor (SCF) and is a member of the subfamily that includes PDGF, CSF-1 and flt-3/flk-2 receptors.
It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes and germ cells.
Upon binding with its ligand, c-Kit receptors are induced undergoing dimerization/ oligomerization and autophosphorylation.
Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCg, p85 subunit of PI3 kinase, SHP2 and CrkL (4), which links c-Kit directly to various cell signaling pathways.
Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors.
Tyr719 is located in the kinase insert region of the catalytic domain.
c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of mouse CD117 protein only when phosphorylated at Tyr719. This antibody crossreacts with tyrosine-phosphorylated PDGF receptor. Species Crossreactivity: human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse c-Kit.
