CD19 is a 95kD coreceptor amplifying the signaling cascade in B cells (1).
On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function.
The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1).
After B cell receptor ligation or CD19 ligation, Tyr513 and Tyr500 of CD19 are progressively phosphorylated.
This enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3).
Coligation of B cell receptor and CD19 also promotes Tyr391 phosphorylation in CD19.
The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).
Applications:Suitable for use in Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of CD19 only when phosphorylated at tyrosine 513. Tyr531 is equivalent to Tyr513 because of the signaling peptide in the CD19 protein sequence.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues around Tyr513 of human CD19.
