CD19 is a 95kD coreceptor amplifying the signaling cascade in B cells (1).
On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function.
The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1).
After B cell receptor ligation or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated.
This enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3).
Coligation of B cell receptor and CD19 also promotes Tyr391 phosphorylation in CD19.
The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Immunocytochemistry (IF): 1:100Flow Cytometry: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and Immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human CD19 independent of its phosphorylated state at 95kD. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IP WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues around the C-terminus of human CD19 (KLH).
