Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1).
It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3).
GSK-3beta may destabilize beta-catenin by phosphorylating it at serines 33, 37 and threonine 41 (4).
Serine 45 in beta-catenin is phosphorylated by CK1.
This likely serves as a priming site for subsequent phosphorylation by GSK-3 (5-7).
Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 (Mr 92kD)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of betacatenin only when phosphorylated at serine 45. It does not recognize beta-catenin phosphorylated at other sites. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Ser45 of human beta-catenin. Species sequence homology: mouse, rat, canine and porcine
