C-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in haematopoietic cells (1,2).
c-Cbl is rapidly tyrosine phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules, such as protein tyrosine kinases, phosphatidylinositol 3 kinase, Crk and 14-3-3 proteins (3,4).
c-Cbl possesses a highly conserved N-terminal phosphotyrosine binding domain (TKB), a C3HC4 RING finger motif.
TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases.
Its RING finger domain recruits ubiquitin-conjugating enzymes.
These two domains are primarily responsible for c-Cbl proteins functioning as ubiquitin protein ligases and downregulation of RTKs (3).
c-Cbl protein in human cancer tissues is frequently tyrosine phosphorylated in a tumor-specific manner (5).
Tyr774 of c-Cbl is phosphorylated in Abl-transformed cells as well as T cells in response to pervanadate and to TcR/CD3-induced stimulation (4,6).
Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects endogenous levels of Tyr774 phosphorylated c-Cbl. The antibody does not crossreact with other tyrosine-phosphorylated proteins.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr774 of human c-Cbl.
