The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1).
The N-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domain.
The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins.
The carboxy-terminal region may be involved in a GTPase-activating function for the small GTP-binding protein Rac (2,3).
The function of Wild-type Bcr in cells remains unclear.
PDGF receptor may use Bcr as a downstrean signal mediator (4).
The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5).
Tyrosine 177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6).
Phosphorylated Tyr177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunohistochemistry (Paraffin): 1:50Flow Cytometry: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human Bcr and Bcr-Abl only when phosphorylated at tyrosine 177. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Tyr177 of human Bcr. Molecular Weight:160kD (Bcr), 210kD (Bcr-Abl).
