The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution.
c-Abl protein is distributed in both the nucleus and the cytoplasm of cells.
It has been implicated in regulation of cell proliferation, differentiation, apoptosis, cell adhesion and stress response.
c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1.
The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood.
Tyrosine 245 is located in the linker region between the SH2 and catalytic domains that is conserved among the Abl family.
Phosphorylation of Tyr245 is involved in activation of c-Abl kinase activity.
Tyrosine 412 is located in the kinase activation loop of c-Abl molecules; phosphorylation of this residue is required for the kinase activity.
Thr735 is in a conserved 14-3-3 protein binding motif, which can be phosphorylated upon stress stimulation or TPA treatment (Wu, J.
et al.
unpublished data).
Phosphorylation of Thr735 in c-Abl may play an important role in regulation of c-Abl localization as well as its function.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol
Specificity：Recognizes endogenouse levels of human c-Abl or Bcr-Ab only when phosphorylated at Thr735. Does not crossreact with other related proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to residues surrounding Thr735 of human c-Abl. Species Sequence Homology: mouse, 100%
