The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene (1).
The N-terminal part of Bcr contains an oligomerization domain, a serine/threonine kinase domain and a region that binds SH2 domain.
The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins.
The C-terminal part may be involved in a GTPase-activating function for the small GTP-binding protein Rac (2,3).
The function of Wildtype Bcr in cells remains unclear.
PDGF receptor may use Bcr as a downstrean signal mediator (4).
The Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML) (5).
Tyrosine 177 of Bcr is phosphorylated in Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl (6).
Phosphorylated tyrosine 177 of Bcr provides a docking site for Gab2 and GRB2 (7,8).
Applications:Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunocytochemistry: 1:100Flow Cytometry: 1:25Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Detects endogenous levels of total Bcr and the 210kD Bcr-Abl fusion protein. Species Crossreactivity: Human, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E FC IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the N-terminal sequence of human Bcr. MW of Antigen: 130, 160kD
