Blk is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2).
Activation of B cells by various ligands is accompanied by activation of Blk (3).
It has been suggested that Blk is involved in the control of B cell differentiation and proliferation (4,5).
Except expressed in B cell, Blk transcripts were also detected in human thymocytes but not in mature T cells, implicating that Blk may play an important role in thymopoiesis (6).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and immunoaffinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes total levels of endogenous human Blk.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding the C-terminus of human Blk (KLH).
