84-1050-72　B Cell Linker Protein, phosphorylated (Tyr96) (BLNK, BASH, BLNK-s, Ly57, SLP-65, SLP65) 100ul　B0397-15

特徴

• BLNK (B cell linker protein) is an adaptor molecule that plays key roles from B cell activation through B cell antigen receptor (BCR) engagent.
• BLNK acts on the inferface between BCR-associated Syk and the downstream signaling cascade (1,2).
• BLNK has multiple SH2 binding motifs (YXXP) at its N-terminus and an SH2 domain at its C-terminus.
• After BCR ligation, BLNK is phosphorylated by Syk on its multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1).
• The phosphorylation on these motifs provides docking sites for signaling molecules such as Btk, PLCgamma and Vav.
• They bind to BLNK through their SH2 domains and cooperatively activate downstream signaling (3,4).
• BLNK can also interact with phosphorylated targets such as HPK1 through its SH2 domain to activate the pathway (5).
• Applications:Suitable for use in Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.