Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1).
The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1 and Chk1.
The essential requirement for the substrates of ATM/ATR is S*/T*Q.
Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases.
Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation.
The complex phenotype of AT cells suggests that it likely has additional substrates.
To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.
Applications:Suitable for use in ELISA, Western Blot and Immunoprecipitation.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:20Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of proteins containing the ATM/ATR substrate motif. Preferentially binds peptides/proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. Does not crossreact with corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. Species Crossreactivity: All species
Isotype：IgG
Calc Applications Abbrev：E IP WB
Immunogen：Synthetic phospho-(Ser/Thr) ATM/ATR substrate peptides, conjugated to KLH
