Apoptosis signal-regulating kinase 1 (ASK1), a MAP kinase kinase kinase, plays essential roles in stress-induced apoptosis (1,2).
ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38 (3).
Overexpression of ASK1 activates JNK and p38 and induces apoptosis through signals involving the mitochondrial cell death pathway in several cell types.
Embryonic fibroblasts or primary neurons derived from ASK1-/-mice are resistant to various stress-induced JNK and p38 activation and cell death (4,5).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1xTBS, and 0.1% Tween-20 at 4ºC overnight with gentle shaking
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes total levels of human overexpressed ASK1 protein independent of its phosphorylation state.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to residues surrounding 280 of human ASK1. Conjugated to KLH.
