An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK.
These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2)
Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis and transcriptional regulation (3-5).
While a number of Akt substrates including GSK-3, Bad and caspase-9 are known, many important substrates await discovery.
Akt phosphory- lates substrates only at serine/threonine in a conserved motif characterized by arginine at positions -5 and -3 (6)
Phospho-Akt substrate-specific antibodies are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg- directed kinases, as well as for high throughput kinase drug discovery.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50Immunohistochemistry (Paraffin): 1:500 using citrate as an unmasking buffer
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some crossreactivity for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. Does not crossreact with the corresponding non-phosphorylated sequences or with other phospho-Ser/Thr-containing motifs. Species Crossreactivity: All
