Akt, also referred to as PKB or Rac, plays a critical role in controlling the balance between survival and apoptosis (1-3).
This protein kinase is activated by insulin and various growth and survival factors, and functions in a wortmannin-sensitive pathway involving PI3 kinase (2,3).
Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and also by phosphorylation within the C-terminus at Ser473.
Akt functions to promote cell survival by inhibiting apoptosis by means of its ability to phosphorylate and inactivate several targets, including Bad (5), Forkhead transcription factors (6) and caspase-9.
PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (7).
LY294002 is a specific PI3 kinase inhibitor (8).
Applications:Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Immunoprecipitation: 1:50Flow Cytometry: 1:50Immunofluorescence (IF-IC): 1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.
Specificity：Detects endogenous levels of total Akt1, Akt2 and Akt3 proteins. Does not crossreact with other related kinases. Species Crossreactivity: human, mouse, rat, hamster, monkey, chicken, D. melanogaster, bovine, porcine, guinea pig
Isotype：IgG
Calc Applications Abbrev：FC IF IP WB
Calc Crossreactivity：Bo Ch Gp Hm Hu Mk Mo Rt
Immunogen：Synthetic peptide (KLH coupled) derived from the C-terminal sequence of mouse Akt. Species sequence homology: canine
