P38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4).
Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far.
Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5).
MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182.
Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).
Applications:Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Immunoprecipitation: 1:50Immunofluorescence/ Immunocytochemistry: 1:100Flow Cytometry: 1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：600ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity：Recognizes endogenous levels of human p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182. Does not crossreact with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Crossreactivity: mouse, rat and monkey
Isotype：IgG
Calc Applications Abbrev：E FC IC IF IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.
