APRIL (a proliferation-inducing ligand) is a newly identified member of the tumor necrosis factor (TNF) family.
Ectopic expression of APRIL is associated with the upregulation of X-linked inhibitor of apoptosis protein (XIAP) and may act as antiapoptotic factor.
Although transcripts of APRIL are of low abundance in normal tissues, high levels of mRNA are detected in transformed cell lines, and in human cancers of colon, thyroid, and lymphoid tissues in vivo.
Applications: Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Direct ELISA: ≥0.5ug/ml using 100ul/well, detects 0.2-04ng/well of recombinant hAPRIL
Western Blot: 0.1-0.2ug/ml.
The detection limit is 1.5-3ng/lane, under either reducing or non-reducing conditions
Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：50ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Specificity：Recognizes human APRIL.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Highly pure (>98%) recombinant human APRIL.
