Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot (Reducing): 1:1000 Cell membrane extracts were examined by SDS-Page, transferred to a nylon membrane.
Membranes were blocked for 1 hour at 4˚C with 0.1% Tween 20 and 2.5% milk powder in PBS.
Membranes were incubated with P1003-05D (1:500 in the same buffer overnight at 4˚C.
Membranes were rinsed and incubated withI1904-47H: IgG, H&L (HRP) Pab GtxRt for 1 hour at RT.
Following rinsing, the membranes were processed using ECLImmunohistochemistry: 1:1000.
Antiserum was used on perfusion fixed tissue.
Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant.
Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
Slide-mounted tissue sections were processed for indirect immunofluorescence.
Slides were incubated with blocking buffer for 1 hour at RT.
P1003-05D was diluted with blocking buffer to the appropriate working concentration.
Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with primary antiserum.
Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at RT.
Slides were again rinsed 3 times and coverslipped.
Staining was examined using fluorescence microscopy.
Immunocytochemistry: 1:1000.
P2X3 transfected cells were processed for indirect immunofluorescence.
Media was removed and cells were gently washed 3 times with serum-free media.
Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：rat
Grade：Serum
Purity：Neat serum.
Form：Supplied as a liquid with 0.05% sodium azide.
Specificity：Recognizes rat P2X2 Receptor. Species Crossreactivity: Human and primate
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：A 13 amino acid peptide Synthetic peptide corresponding to amino acids 460-472 (DSTSDPKGLAQL) from the carboxy-terminus of the rat P2X2 Receptor protein.
