84-1050-42　P2X1 Receptor 50ul　P1003-01

特徴

• Applications:Suitable for use in Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:1000 Antiserum was used on perfusion fixed tissue.
• Perfusion: 1) calcium-free Tyrode’s solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant.
• Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
• Slide-mounted tissue sections were processed for indirect immunofluorescence.
• Slides were incubated with blocking buffer for 1 hour at room temperature.
• Primary antiserum was diluted with blocking buffer to the appropriate working concentration.
• Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with primary antiserum.
• Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature.
• Slides were again rinsed 3 times and coverslipped.
• Staining was examined using fluorescence microscopy
• Immunocytochemistry: 1:1000 P2X1 transfected cells were processed for indirect immunofluorescence.
• Media was removed and cells were gently washed 3 times with serum-free media.
• Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
• Note: Sodium azide (NaN 3) interferes with peroxidase reactions and should not be used with peroxidase methodologies.
• If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.