Metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors activated by glutamate.
Based on sequence similarity, transduction mechanisms and agonist potencies, mGluRs are subdivided into three groups: mGluR1/mGluR5, mGluR2/mGluR3, and mGluR4/mGluR6/mGluR7/mGluR8.
mGluRs are widely distributed throughout the nervous system and are expressed by both neurons and glial cells.
mGluRs have been suggested to play a variety of functional roles, among which is involvement in synaptic plasticity underlying learning and memory as well as chronic pain.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500.
Only shown to work in systems (transfected cells) with high expression levels uning standard protocols
Immunohistochemistry: 1:1000.
Sodium azide interferes with peroxidase reactions and should not be used with peroxidase methodologies.
If sodium azide is present in any steps of the staining procedure, the tissue should thoroughly be rinsed with sodium azide-free buffer before performing the peroxidase reaction
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris-glycine, pH 7.8, 1% BSA, 0.09% sodium azide.
Specificity：Recognizes rat mGluR3.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Rt
Immunogen：Synthetic peptide corresponding to aa118-126, TKVDEAEYM of rat mGluR3. Species sequence homology: mouse and human: 100%.
