84-1050-33　Endomorphin 1 50ul　E2291-02

特徴

• Endomorphins are recently discovered endogenous opioid peptides composed of four amino acids: Endomorphin-1 (EM-1) is Tyr-Pro-Trp-Phe-NH2, and Endomorphin-2 (EM-2) is Tyr-Pro-Phe-Phe-NH2.
• These peptides have a very high affinity and specificity for mu-opioid receptors as it has been reported in many pharmacological studies.
• EM-1 is distributed in many brain regions, whereas EM-2 is prevalent in brainstem and spinal cord.
• EM-1 and EM-2 exert their antinociceptive activity by interacting with mu-opioid receptors.
• EM-1 anf EM-2 bind with mu-opioid receptor with high affinity and selectivity in comparison with binding to opioid delta and kappa receptors.
• EM-1 and EM-2 tetrapeptides activate G-proteins and inhibit adenylyl cyclase activity when bind to mu-opioid receptors.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry (Immunofluorescence): 15mg/mlImmunohistochemistry (Avidin-Biotin): 5mg/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O, 0.1% sodium azide.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.