84-1050-29　［取扱停止］Capsaicin Receptor (VR1, Transient receptor potential cation channel subfamily V member 1, TrpV1, osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid receptor type 1-like) 50ul　C1078-25

特徴

• VR1 (vanilloid receptor 1) is a channel activated by capsaicin, the pungent ingredient in hot peppers.
• It was isolated from sensory neurons.
• Sequence analysis suggests that VR1 has six transmembrane domains and intracellular N- and C-termini.
• It shares structural similarities with the family of putative store-operated calcium channels.
• VR1 is expressed by nociceptive sensory neurons.
• It is activated by noxious heat and is thought to function as transducer of noxious thermal stimuli.
• Applications:Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Indirect Immunofluorescence.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000 Immunohistochemistry: 1:1000.
• Used on perfusion fixed tissue.
• Perfusion: 1) calcium-free Tyrode’s solution, 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant.
• Desired tissues were dissected and stored overnight in 10% sucrose in PBS.
• Slide-mounted tissue sections were processed for indirect immunofluorescence.
• Slides were incubated with blocking buffer for 1 hour at RT.
• C1078-25 was diluted.
• Blocking buffer was removed and slides were incubated for 18-24 hours at 4°C with C1078-25.
• Slides were rinsed 3x and then incubated with secondary antibodies for 1 hour at RT.
• Slides were again rinsed 3x and coverslipped.
• Staining was examined using fluorescence microscopy.
• Immunocytochemistry: 1:1000.
• VR1 transfected cells were processed for indirect Immunofluorescence.
• Media was removed and cells were gently washed 3x with serum-free media.
• Following fixation, cells were processed for indirect Immunofluorescence as described above
• Note: sodium azide interferes with peroxidase reactions and should not be used with peroxidase methodologies.
• If sodium azide is present in any steps of the stainig procedure, the tissue should be thouroughly washed with sodium-free buffer before performing the peroxidase reaction
• Optimal dilutions to be determined by the researcher.
• Control peptide:C1078-25A: Capsaicin Receptor, NT, Control Peptide (Vanilloid Receptor 1, VR1)
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.